The church of Saint Francis is one of the most important monuments of Rethymno. It was the main temple of the Monastery of the Franciscan Order. The architecture of the building - a single aisle Basilica type with wooden roof - and its ornaments are very interesting. Next to the east side of the temple two deserted chapels are preserved. Its Corinthian style main doorway is impressive, with capitals of composite order. During recent excavations around the church valuable archeological findings have been discovered, including two tombs of Venetian nobles.
During the Turkish occupation the temple was turned into an "Imaret" (a shelter for the poor).
It was also used as a cultural center until 1996. It was recently renovated to accommodate the Byzantine and Post-Byzantine Collection of the Prefecture of Rethymno.

The Guora Gate (Porta Guora or Grand Gate)) is the main entrance of the Venetian city walls that protected the city of Rethymno. The gate was built in the years of Rector Rettore Jocopo Guoro (1566-1568). Part of the gate is preserved at the beginning of the street "Ethnikis Antistaseos". It is 2,60 m. wide, built with carved stones with skew acnes, creating a semicircular arc on the top.
According to traveler J. Gerola, the initial shape of the gate was formed by stepped cornice and pediment where a relief the lion of St. Marcos (the Venetian emblem) existed. Today the relief is preserved in two pieces and is stored at Loggia's courtyard.
Old drawings of Guora Gate: inside the walls - outside the walls

The Historical and Folklore Museum of Rethymno is located next to the Neratze Mosque. It is an institution of public benefit, founded in 1973 by the President of the Historical and Folklore Institution, Christoforos Stavroulakis, and Fali Vogiatzaki. The museum is housed in a restored Venetian building with an interior courtyard. The building is a wonderful piece of urban residence of the last phase of the Venetian occupation of Crete, built in the renaissance style by traditional craftsmen.

The square of Mikrasiaton (GR: Πλατεία Μικρασιατών) formerly the 1st primary school square, in Rethymnon town, covers an area of 7,500 square meters, right at the heart of the city's historical center. It is surrounded by valuable monuments and beautiful buildings, mainly used as cultural centers.
There, visitors have the opportunity to rest in a peaceful environment and at the same time visit the various monuments and cultural centers located in the area.

The Museum of Submarine Life is situated at Arapatzoglou street, at the center of Rethymno's old city. It was founded by the "Moshaki" family, in memory of their lost child, who drowned in the sea. The largest part of the exhibits are made up of shells, while a few vertebrates, sponges and fish are also on display.